Hypoxia-induced angiogenesis and capillary density determination.

Methods in molecular biology (Clifton, N.J.)

PubMedID: 24510855

Tsipis CP, Sun X, Xu K, LaManna JC. Hypoxia-induced angiogenesis and capillary density determination. Methods Mol Biol. 2014;113569-80.
Chronic exposure to moderate hypoxia elicits structural and functional changes in the microvascular network of the mammalian CNS. Hypoxia-induced angiogenesis can be elicited and studied by a relatively simple experimental method. Rats or mice can be exposed to mild hypoxia in a hypobaric chamber, or alternatively in a normobaric hypoxia chamber. After 3 weeks, the animals are perfused and fixed, the brain removed, and paraffin embedded and sectioned at 5 ┬Ám. The sections are stained for the blood-brain barrier glucose transporter (GLUT-1) by immunohistochemistry, capillary profiles identified and counted as a measure of angiogenesis.