Electroporation-mediated gene delivery to the lungs.

Methods in molecular biology (Clifton, N.J.)

PubMedID: 24510824

Young JL, Barravecchia MS, Dean DA. Electroporation-mediated gene delivery to the lungs. Methods Mol Biol. 2014;1121189-204.
Electroporation is a safe, efficient, and inexpensive method to transfer naked plasmid DNA into various tissues. For electroporation-mediated gene transfer to the mouse lung, a plasmid solution is delivered to the lungs via the trachea. Immediately after plasmid delivery, eight square wave pulses are delivered by two pre-gelled electrodes placed on each side of the chest. An optimal field strength in mice is 200 V/cm, with a pulse duration of 10 ms each and a 1 s interval between pulses. High level gene expression can be achieved within 24 h in all cell types in the lung with very little inflammation and no apparent trauma.