Novel description of mcl-PHA biosynthesis by Pseudomonas chlororaphis from animal-derived waste.

Journal of biotechnology

PubMedID: 23467001

Muhr A, Rechberger EM, Salerno A, Reiterer A, Malli K, Strohmeier K, Schober S, Mittelbach M, Koller M. Novel description of mcl-PHA biosynthesis by Pseudomonas chlororaphis from animal-derived waste. J Biotechnol. 2013;165(1):45-51.
A novel description of mcl-PHA biosynthesis by Ps. chlororaphis from tallow-based biodiesel as an inexpensive carbon feed stock is presented. Fermentation protocols, kinetic analysis, an efficient product recovery strategy, and product characterization are included. Maximum specific growth rates (┬Ámax.) of 0.08 h(-1), 0.10 h(-1) and 0.13 h(-1), respectively, were achieved in three different fermentation set-ups. Volumetric productivity for mcl-PHA amounted to 0.071 g/L h, 0.094 g/L h and 0.138 g/L h, final intracellular PHA contents calculated from the sum of active biomass and PHA from 22.1 to 29.4 wt.-%, respectively. GC-FID analysis showed that the obtained biopolyester predominantly consists of 3-hydroxyoctanoate and 3-hydroxydecanoate, and, to a minor extent, 3-hydroxydodecanoate, 3-hydroxynonanoate, 3-hydroxyhexanoate, and 3-hydroxyheptanoate monomers. The overall distribution of the monomers remained similar, regardless to working volumes, biodiesel concentrations and pre-treatment of the inoculum.