Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification.

Chemical communications (Cambridge, England)

PubMedID: 24525625

Hong CY, Chen X, Li J, Chen JH, Chen G, Yang HH. Direct detection of circulating microRNAs in serum of cancer patients by coupling protein-facilitated specific enrichment and rolling circle amplification. Chem Commun (Camb). 2014;.
We have developed a simple method for direct detection of circulating microRNAs in serum by using the p19 protein-functionalized magnetic beads (PFMBs) for specific enrichment and rolling circle amplification (RCA). The detection limit for microRNAs is 1 fM. Therefore, the proposed method has the potential of being used in the analysis of circulating microRNAs and clinical diagnosis.