Altered Fecal Microbiota Composition for Food Allergy in Infants.

Applied and environmental microbiology

PubMedID: 24532064

Ling Z, Li Z, Liu X, Cheng Y, Luo Y, Tong X, Yuan L, Wang Y, Sun J, Li L, Xiang C. Altered Fecal Microbiota Composition for Food Allergy in Infants. Appl Environ Microbiol. 2014;.
Increasing evidence suggests that perturbations in the intestinal microbiota composition of infants may be implicated in the pathogenesis of food allergy (FA), while its actual structure and composition in human beings with FA remains unclear. Microbial diversity and composition were analyzed with parallel barcoded 454 pyrosequencing targeting the 16S rRNA gene hypervariable V1-V3 regions in the feces of 34 infants with FA (17 IgE-mediated and 17 non-IgE-mediated) and 45 healthy controls. Here we showed that several key FA-associated bacterial phylotypes, but not the overall microbiota diversity, significantly changed in infancy fecal microbiota with FA and were associated with the development of FA. The proportion of abundant Bacteroidetes, Proteobacteria, and Actinobacteria phyla were significantly reduced, while the Firmicutes phylum was highly enriched in the FA group (P<0.05). Abundant Clostridiaceae 1 was prevalent in FA infants at the family level (P=0.016). FA-enriched phylotypes negatively correlated with interleukin 10, for example the genus Enterococcus and Staphylococcus. Despite profound interindividual variability, 20 predominant genera was significantly different between the FA and healthy control groups (P<0.05). Infants with IgE-mediated FA had increased Clostridium sensu stricto and Anaerobacter and decreased Bacteroides and Clostridium XVIII (P<0.05). A positive correlation was observed between Clostridium sensu stricto and serum-specific IgE (R=0.655, P<0.001). The specific microbiota signature could distinguish infants with IgE-mediated FA from non-IgE-mediated ones. Detailed microbiota analysis of a well-characterized cohort of infants with FA showed that dysbiosis of fecal microbiota with several FA-associated key phylotypes may play a pathogenic role in FA.