Cellular Mechanisms of a New Pyrazinone Compound that Induces Apoptosis in SKOV-3 Cells.

Asian Pacific journal of cancer prevention : APJCP

PubMedID: 24568498

Wang G, Jiang MY, Meng Y, Song HR, Shi W. Cellular Mechanisms of a New Pyrazinone Compound that Induces Apoptosis in SKOV-3 Cells. Asian Pac J Cancer Prev. 2014;15(2):797-802.
We screened a small molecular library that was designed and independently synthesized in vitro and found a new drug (MY-03-01) that is active against ovarian cancer. We established that MY-03-01 effectively inhibited SKOV-3 cell survival in a dose-dependent manner, based on cell viability rates, and that it not only induced SKOV-3 apoptosis by itself, but also did so synergistically with paclitaxel. Secondly, when MY-03-01 was applied at 40 ┬ÁM, its hemolytic activity was less than 10%, compared with the control, and there was almost no damage to normal cells at this concentration. In addition, we used DAPI staining and flow cytometry to show that MY- 03-01 could significantly induce apoptosis of SKOV-3 cells. Finally, we found that MY-03-01 likely induced SKOV-3 apoptosis by activating caspase3 and caspase9 through the mitochondrial pathway.