Effects of Angiopoietin-1 on Inflammatory Injury in Endothelial Progenitor Cells and Blood Vessels.

Current gene therapy

PubMedID: 24606182

Wang YQ, Song JJ, Han X, Liu YY, Wang XH, Li ZM, Tzeng CM. Effects of Angiopoietin-1 on Inflammatory Injury in Endothelial Progenitor Cells and Blood Vessels. Curr Gene Ther. 2014;.
Endothelial progenitor cells (EPCs) and angiopoietin-1 (Ang-1) play important roles for vasculogenesis and angiogenesis, respectively. Targeting both aspects of cardiovascular tissue regeneration may offer promising therapeutic options for cardiovascular disorders. To this end, we constructed a lentiviral vector (pNL) with the Ang-1 gene and transfected EPCs with it (Ang-1-EPCs) to investigate vasculogenesis in both cellular and animal models. As compared to controls, intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) were increased significantly in both untreated EPCs and in a pNL vector group. After Ang-1 transcription, ICAM-1 and VCAM-1 were decreased considerably in those treatment groups. Ang-1-Modified EPCs alleviated inflammatory responses induced by tumor-necrosis factor-a (TNF-a) in vitro. Moreover, Ang-1-EPC implantation inhibited neointimal hyperplasia after balloon catheter injury in rats, dramatically suppressing the intimal-media (I/M) ratio and decreasing this size of the neointimal area. Proliferating cell nuclear antigen (PCNA) expression in the Ang-1-EPC group was lower than the EPC non-treatment group also, suggesting that Ang-1-EPC improved cell survival during inflammation and promoted endothelialization in damaged blood vessels.