Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts.

International journal of biological macromolecules

PubMedID: 23261417

Tao S, Gao S, Zhou Y, Cao M, Xie W, Zheng H, Fan L. Preparation of carboxymethyl chitosan sulfate for improved cell proliferation of skin fibroblasts. Int J Biol Macromol. 2013;54160-5.
Carboxymethyl chitosan (CMC) was chemically modified by sulfating agent N(SO(3)Na)(3). The product carboxymethyl chitosan sulfate (CMCS) was characterized by Fourier transform infrared spectroscopy (FT-IR) and carbon-13 nuclear magnetic resonance spectroscopy ((13)C NMR). And the optimal reaction conditions were also studied by means of single factor experiment. MTT method was applied to evaluate the effects of CMCS on proliferation of skin fibroblasts. The results revealed the CMCS with sulfate content of 26.26% at the concentration of 100 µg/ml best promoted the proliferation of skin fibroblasts.