Isolation and characterization of hyaluronic acid from the liver of marine stingray Aetobatus narinari.

International journal of biological macromolecules

PubMedID: 23220595

Sadhasivam G, Muthuvel A, Pachaiyappan A, Thangavel B. Isolation and characterization of hyaluronic acid from the liver of marine stingray Aetobatus narinari. Int J Biol Macromol. 2013;5484-9.
Although hyaluronic acid research pursuits ahead in exploring its biomedical perspective, very limited investigations were carried out in their isolation shape view point, furthermore, most of the investigations were targeted towards the terrestrial source. To swerve from that, the present study was projected through the marine superstore, where in high molecular weight hyaluronic acid of 13, 65,863 Da was isolated from the liver of stingray Aetobatus narinari. The purified HA was confirmed at the preliminary level by their stains all dye binding nature. Their analytical composition including carbon, hydrogen, nitrogen, N-acetyl glucosamine, glucuronic acid contents was analysed. The HA was characterized by agarose-gel electrophoresis, FTIR, HPTLC, and (1)H NMR. The DPPH radical scavenging activity of HA and its reducing power was evident to all the tested concentrations, but lower than that of ascorbic acid. HA showed significant inhibition against the proliferation of cells, substantiating its influence in regulation of cell functions.