Structure of the O-polysaccharide of Edwardsiella tarda PCM 1150 containing an amide of D-glucuronic acid with L-alanine.

Carbohydrate research

PubMedID: 23348241

Katzenellenbogen E, Kocharova NA, Shashkov AS, Górska-Fraczek S, Bogulska M, Gamian A, Knirel YA. Structure of the O-polysaccharide of Edwardsiella tarda PCM 1150 containing an amide of D-glucuronic acid with L-alanine. Carbohydr Res. 2013;36884-8.
Mild acid degradation of the lipopolysaccharide of Edwardsiella tarda PCM 1150 afforded an O-polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopies, including experiments performed in a 9:1 H2O/D2O mixture to detect NH protons and their correlations with CH protons. The O-polysaccharide was found to contain an amide of d-glucuronic acid with l-alanine (d-GlcA6Ala) and the following structure of the branched hexasaccharide repeating unit was established: -->4)-ß-D-GlepA6Ala-(1-->4)-a-L-Fucp-(1-->4)-a-D-Glcp-(1-->4)-a-D-Quip-(1-->3)-ß-D-GlcpNAc-(1-->3<--1a-D-GalpNAc.