Insulin-loaded PLGA microparticles: flow focusing versus double emulsion/solvent evaporation.

Journal of microencapsulation

PubMedID: 21736527

Cózar-Bernal MJ, Holgado MA, Arias JL, Muñoz-Rubio I, Martín-Banderas L, Alvarez-Fuentes J, Fernández-Arévalo M. Insulin-loaded PLGA microparticles: flow focusing versus double emulsion/solvent evaporation. J Microencapsul. 2011;28(5):430-41.
CONTEXT
Oral administration of insulin is severely limited by very low bioavailability. Biocompatible polymeric nanocarriers have been investigated to overcome this problem. Flow focusing (FF) has revolutionized current engineering of poly(D,L-lactide-co-glycolide) (PLGA) based micromedicines. This technique has never been used to formulate insulin-loaded PLGA microparticles.

OBJECTIVE
Investigation of the benefits rising from the synthesis of insulin-loaded PLGA microplatforms by FF, compared to double emulsion/solvent evaporation method.

MATERIALS AND METHODS
Both synthesis methodologies were compared in terms of geometry, surface physicochemical properties and insulin vehiculization capabilities. The stability of the peptide during the formulation procedure was further analysed.

RESULTS
FF permitted the preparation of insulin-loaded microcarriers with better geometry and physicochemical properties for the oral route, along with greater insulin loading capabilities and sustained insulin release kinetics.

DISCUSSION AND CONCLUSION
Results have lead to the identification of the best formulation conditions for the engineering of insulin-loaded PLGA microparticles against diabetes.