Organization of the Y chromosome in testis cells of fetal, subadult and adult mice as determined by in situ hybridization.

Chromosoma

PubMedID: 8306823

Guttenbach M, Winking H, Schmid M. Organization of the Y chromosome in testis cells of fetal, subadult and adult mice as determined by in situ hybridization. Chromosoma. 1993;102(9):618-22.
In order to reveal the time-course of decondensation of the Y chromosome in Sertoli cells, testes preparations of fetal, subadult and adult laboratory mice in different developmental stages were hybridized in situ with biotinylated probe pY353/B, which binds along the entire long arm of the mouse Y chromosome. All fetal and subadult testicular cells exhibited tightly compacted hybridization signals, indicating highly condensed Y chromosomes. Diffuse signals, indicating decondensation of the Y chromosome were found for the first time in the structurally differentiated Sertoli cells of 35 to 40 day old animals. Since this coincides with the appearance of mature sperm nuclei, a correlation between decondensation of the Y chromosome and its activity in sperm maturation and/or sperm motility can be presumed.