EGR1 supports the osteogenic differentiation of dental stem cells.

International endodontic journal

PubMedID: 24749562

Press T, Viale-Bouroncle S, Felthaus O, Gosau M, Morsczeck C. EGR1 supports the osteogenic differentiation of dental stem cells. Int Endod J. 2014;.
AIM
To evaluate whether and how the transcription factor early growth response gene 1 (EGR1) affects the osteogenic differentiation of dental stem cells.

METHODOLOGY
Dental stem cells from apical papilla (SCAPs) and from the dental follicle (DFCs) were transfected with EGR1 specific siRNA or EGR-1 expression plasmid. Gene regulation was verified at protein level by Western blotting. The expression of the transcription factors distal-less homeobox 3 (DLX3), alkaline phosphatase (ALP), and bone morphogenetic protein 2 (BMP2), which are all regulators and markers of the osteogenic differentiation in dental stem cells, was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). To investigate the mineralization, SCAP long term cultures were stained with alizarin red after EGR1 over-expression.

RESULTS
EGR1 was induced in SCAPs during osteogenic differentiation. DLX3 and bone morphogenetic protein 2 (BMP2) were up-regulated after EGR1 over-expression and down-regulated after EGR1 depletion. The expression of ALP was also down-regulated after EGR1 depletion. The over-expression of EGR1 in SCAPs promoted mineralization after osteogenic differentiation.

CONCLUSIONS
EGR1 supported the osteogenic differentiation of dental stem cells by potentially regulating the expression of DLX3 and BMP2. This article is protected by copyright. All rights reserved.