Restriction fragment length polymorphism of the caprine beta-globin gene cluster associated with the Hb beta-globin variants in Norwegian dairy goats.

Animal genetics

PubMedID: 1673827

Bergersen O, Braend M. Restriction fragment length polymorphism of the caprine beta-globin gene cluster associated with the Hb beta-globin variants in Norwegian dairy goats. Anim Genet. 1991;22(1):53-65.
Restriction fragment length polymorphism (RFLP) analysis was used to characterize the beta-globin cluster in Norwegian dairy goats. In 122 individuals, different RFLP patterns were detected after BglII and PstI digestion and hybridization with a caprine beta-globin probe. The location of the polymorphic BglII and PstI sites were determined. The different RFLPs were in linkage disequilibrium with each other and with the beta-globin locus as studied at the protein level. A preferential association between certain RFLP haplotypes and beta-globin variants was observed. Polymorphic DNA fragments in the epsilon-globin genes were detected after MspI digestion and hybridization with a caprine epsilon-probe, and eight different band patterns were observed. Correlation between different MspI haplotypes and the beta-globin variants was determined.