c-myc mRNA in cytoskeletal-bound polysomes in fibroblasts.

The Biochemical journal

PubMedID: 2006923

Hesketh JE, Campbell GP, Whitelaw PF. c-myc mRNA in cytoskeletal-bound polysomes in fibroblasts. Biochem J. 1991;274 ( Pt 2)607-9.
3T3 fibroblasts were treated sequentially with 25 mM-KCl/0.05% Nonidet P40, 130 mM-KCl/0.05% Nonidet P40 and finally with 1% Nonidet P40/1% deoxycholate in order to release free, cytoskeletal-bound and membrane-bound polysomes respectively. The membrane-bound fraction was enriched in the mRNA for the membrane protein beta 2-microglobulin, whereas the cytoskeletal-bound polysomes were enriched in c-myc mRNA. Actin mRNA was present in both free and cytoskeletal-bound polysomes. The results suggest that cytoskeletal-bound polysomes are involved in the translation of specific mRNA species.