Inhibition of lysenin-induced hemolysis by all-E-lutein derived from the plant Dalbergia latifolia.

Planta medica

PubMedID: 22588837

Niwa Y, Matsui C, Sukumwang N, Iinuma H, Ikeda Y, Koyano T, Kovitayakorn T, Simizu S, Umezawa K. Inhibition of lysenin-induced hemolysis by all-E-lutein derived from the plant Dalbergia latifolia. Planta Med. 2012;78(10):957-61.
Lysenin is a pore-forming toxin derived from coelomic fluid of the earthworm Eisenia foetida. The model of lysenin-induced hemolysis includes the specific binding of lysenin to sphingomyelin, oligomerization of the pore proteins, and pore formation. Although the mechanism of lysenin-induced hemolysis is unique, its precise mechanism of action and its inhibitors are poorly understood. In the present study, we screened for inhibitors of lysenin-induced hemolysis by using an optimized screening system and found a methanolic extract of Dalbergia latifolia leaves to be a potential candidate. After isolation and identification, all-E-lutein was identified as the hemolysis inhibitor with an effective dose of 0.025-2.5 ng/mL without any toxicity. The inhibition by all-E-lutein is likely to occur during the receptor binding and/or pore-forming protein oligomerization.