ATP-stimulated glutamate-dependent calcium uptake by rat synaptosomes.

Clinica chimica acta; international journal of clinical chemistry

PubMedID: 1572076

Galzigna L, Bianchi M, Battistin T, Scarpa M, Rigo A. ATP-stimulated glutamate-dependent calcium uptake by rat synaptosomes. Clin Chim Acta. 1992;206(1-2):147-53.
The entry of Ca2+ in rat synaptosomes was followed with a Ca(2+)-selective electrode. Extracellular ATP is necessary for the entry which is a function of synaptosomal protein, free Ca2+ and glutamate concentrations. Ketamine, glycine and kainate have negligible effect while quisqualate slightly inhibits the uptake of Ca2+ in the presence of glutamate. The added ATP is hydrolyzed by the synaptosomes through an ouabain-insensitive ecto-ATPase affected by the presence of Ca2+, glutamate and, to a slight extent, NMDA.