Interleukin 17A Promotes Gastric Cancer Invasiveness via NF-?B Mediated Matrix Metalloproteinases 2 and 9 Expression.

PloS one

PubMedID: 24905806

Wang Y, Wu H, Wu X, Bian Z, Gao Q. Interleukin 17A Promotes Gastric Cancer Invasiveness via NF-?B Mediated Matrix Metalloproteinases 2 and 9 Expression. PLoS ONE. 2014;9(6):e96678.
Interleukin 17A (IL-17A), as a pro-inflammatory cytokine, is involved in pathology of inflammatory diseases and tumor microenvironment. The aim of this study is to investigate the effect of IL-17A on the invasiveness of gastric cancer (GC). In the study, we found that IL-17A could promote the migration and invasion of GC cells. Furthermore, after treated with IL-17A, the expressions and activities of matrix metalloproteinase 2 (MMP-2) and MMP-9 were upregulated, while the expressions of TIMP-1 and TIMP-2 were downregulated. Moreover, the nuclear/overall fractions of p65 and p50 were dramatically elevated by IL-17A. Pretreatment with helenalin, a nuclear factor-?B (NF-?B) inhibitor, was proved to abolish the promoting effect of IL-17A on the invasion ability of GC cells and upregulation of MMP-2 and MMP-9. In conclusion, our findings illustrated that IL-17A could promote the invasion of GC cells by activating NF-?B pathway, and subsequently upregulating the expression of MMP-2 and MMP-9. These results may lead to the identification of new diagnostic markers and therapeutic targets of GC.