CD4(+)CXCR5(+) follicular helper T cells in salivary gland promote B cells maturation in patients with primary Sjogren's syndrome.

International journal of clinical and experimental pathology

PubMedID: 24966908

Jin L, Yu D, Li X, Yu N, Li X, Wang Y, Wang Y. CD4(+)CXCR5(+) follicular helper T cells in salivary gland promote B cells maturation in patients with primary Sjogren's syndrome. Int J Clin Exp Pathol. 2014;7(5):1988-96.
OBJECTIVE
To examine amount of CD4(+)CXCR5(+)Tfh cells and B cells subsets in salivary gland and peripheral blood from patients with primary Sjogren's syndrome (pSS) and to analyze whether the frequency of CD4(+)CXCR5(+)Tfh cells is associated with pSS pathologic process.

METHODS
The percentages of CD4(+)CXCR5(+)Tfh cells and B cell subsets were examined by flow cytometry. B-lymphocyte chemoattraetant (BLC; also called CXCL13), IL-21, IL-6 from the serum of pSS patients was assessed by polymerase chain reaction-enzyme-linked immunosorbent assay (ELISA).

RESULTS
The percentages of CD4(+)CXCR5(+)Tfh cells in peripheral blood were increased in pSS patients, but decreased after treatment with glucocorticoid and/or immunosuppressive drugs. Abnormal B cell subsets appeared in salivary and peripheral blood of pSS patients. The frequency of salivary CD4(+)CXCR5(+)Tfh cells was positively correlated with CD19(+)CD27(+) memory B cells and CD19(+)CD27(high) plasma cells. Also increase of salivary CD19(+)CD27(high) plasma cells was positively associated with serum ANA titer of pSS patients.

CONCLUSIONS
CD4(+)CXCR5(+)Tfh cells are significantly increased in salivary and peripheral blood in pSS patients with aberrant CD19(+)CD27(+) memory B cells and CD19(+)CD27(high) plasma cells, suggesting that CD4(+)CXCR5(+)Tfh cells may contribute to the pathogenesis of pSS by promoting the maturation of B cells.