Citrate Crosslinked Gels with Strain Reversibility and Viscoelastic Behavior Accelerates Healing of Osteochondral Defects in Rabbit Model.

Langmuir : the ACS journal of surfaces and colloids

PubMedID: 24971647

Ghosh P, Rameshbabu AP, Dhara S. Citrate Crosslinked Gels with Strain Reversibility and Viscoelastic Behavior Accelerates Healing of Osteochondral Defects in Rabbit Model. Langmuir. 2014;.
Most living tissues are viscoelastic in nature. Self-repair due to dissipation of energy by reversible bonds prevents rupture of molecular backbone in these tissues. Recent studies therefore, aim to synthesize biomaterials that approximate mechanical performance of biological materials with self-recovery property. We report an environmentally friendly method for development of ionotropically crosslinked viscoelastic chitosan gels with modulus comparable to living tissues. The strain recovery property was found to be highest for the gels with lowest crosslinking density. Force-displacement curve showed significant hysteresis due to presence of reversible bonds in the crosslinked gels. Nanoindentation studies demonstrated creep phenomenon for the crosslinked chitosan gels. Creep, hysteresis and plasticity index confirmed viscoelastic behavior of the crosslinked gels. The viscoelastic gels were implanted at osteochondral defect sites to assess tissue regeneration ability. In vivo results demonstrated early cartilage formation and woven bone deposition for defects filled with the gels compared to non-treated defects.