CD34+ Eosinophil-Lineage-Committed Cells in the Mouse Lung.

Methods in molecular biology (Clifton, N.J.)

PubMedID: 24986605

Bossios A, RĂ¥dinger M. CD34+ Eosinophil-Lineage-Committed Cells in the Mouse Lung. Methods Mol Biol. 2014;117829-43.
Several studies suggest that eosinophil progenitor cells are capable of extramedullary proliferation but also enhance chronic inflammation via their own production of inflammatory and chemotactic mediators, thus augmenting the degree of inflammation by recruitment of more progenitors or mature effector cells, such as eosinophils at the site of inflammation. In this chapter, we provide methods focused on detecting eosinophil progenitor cells in the lung of allergen-challenged mice and how to monitor their proliferation capacity.