GTPases Rho distribution in intraepithelial and invasive neoplasias of the uterine cervix.

European journal of gynaecological oncology

PubMedID: 24984542

Tibúrcio MG, Pinheiro NM, Carboni Sde S, Rocha LP, Adad SJ, Maluf PJ, Murta EF, Crema VO. GTPases Rho distribution in intraepithelial and invasive neoplasias of the uterine cervix. Eur J Gynaecol Oncol. 2014;35(3):284-8.
PURPOSE OF INVESTIGATION
To evaluate the distribution of GTPases RhoA, RhoB, and Cdc42 in cervical intraepithelial neoplasias (CIN) and invasive neoplasias of the uterine cervix.

MATERIALS AND METHODS
samples of neoplastic lesions of the uterine cervix of 44 patients were classified in: CIN I (n = 10), CIN II (n = 10), CIN III (n = 09), and invasive carcinoma (n = 15). Antibodies anti-RhoA, anti-RhoB, and anti-Cdc42 were used and staining was classified as: negative, mild, moderate, and intense positive.

RESULTS
When compared with dysplastic cells, superficial cells showed: higher expression of RhoB in CIN I (p = 0.0018), and lower expression of Cdc42 in CIN I (p = 0.0225). The authors observed higher expression of RhoA (p = 0.0002) and RhoB (p = 0.0046) in CIN dysplastic cells when compared with invasive carcinoma cells.

CONCLUSIONS
GTPases Rho may be involved with the regulation of biological processes, important to the progression of cervical neoplasias. Probably, RhoA is important for maintenance of cell differentiation and RhoB protects cells from malignant cervical neoplasia.