P400Effects of a sucrose-enriched diet on the pattern of gene expression, contraction and Ca2+ transport in Goto-Kakizaki type 2 diabetic rat heart.

Cardiovascular Research

PubMedID: 25020786

Howarth F, El Nebrisi E, Jayaprakash P, Qureshi M, Parekh K, Oz M, Adrian T. P400Effects of a sucrose-enriched diet on the pattern of gene expression, contraction and Ca2+ transport in Goto-Kakizaki type 2 diabetic rat heart. Cardiovasc Res. 2014;103 Suppl 1S74.
There has been a spectacular rise in the global prevalence of type 2 diabetes mellitus and cardiovascular disease is the major cause of morbidity and mortality in diabetic patients. A variety of diastolic and systolic dysfunctions have been demonstrated in type 2 diabetic heart. The consumption of sugar-sweetened beverages has been linked to rising rates of obesity which in turn is a risk factor for development of type 2 diabetes mellitus. The effects of a sucrose-enriched diet on the pattern of gene expression, contraction and calcium transport in the Goto-Kakizaki (GK) type 2 diabetic rat heart have been investigated. Genes encoding cardiac muscle proteins (Myh7, Mybpc3, Myl1, Myl3, Mylpf), intercellular proteins (Gja4), cell membrane transport (Atp1b1), calcium channels (Cacna1c, Cacna1g, Cacnb1), potassium channels (Kcnj11) were upregulated and genes encoding potassium channels (Kcnb1) were downregulated in GK compared to Control rats. Genes encoding cardiac muscle proteins (Myh6, Mybpc3, Tnn2), intercellular proteins (Gja1, Gja4), intracellular calcium transport (Atp2a1, Ryr2), cell membrane transport (Atp1a2, Atp1b1), potassium channel (Kcnj2, Kcnj8) proteins were upregulated and genes encoding cardiac muscle proteins (Myh7) were downregulated in Control/Sucrose compared to Control rats. Genes encoding cardiac muscle proteins (Myh7), potassium channel (Kcnj11) proteins were downregulated in GK/Sucrose compared to Control rats. Amplitude of shortening was reduced in myocytes from Control/Sucrose compared to Control and in GK/Sucrose compared to GK rats. Amplitude of the calcium transient was increased in myocytes from Control/Sucrose compared to Control and decreased in GK/Sucrose compared to GK rats. Subtle alterations in the pattern of expression of genes encoding a variety of cardiac muscle proteins are associated with changes in shortening and intracellular calcium transport in ventricular myocytes from GK type 2 diabetic and Control rats fed a sucrose-enriched diet.