The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier.

Proceedings of the National Academy of Sciences of the United States of America

PubMedID: 5264138

Benemann JR, Yoch DC, VALENTINE RC, Arnon DI. The electron transport system in nitrogen fixation by Azotobacter. I. Azotoflavin as an electron carrier. Proc Natl Acad Sci USA. 1969;64(3):1079-86.
A flavoprotein, named azotoflavin, was isolated from an extract of Azotobacter vinelandii cells, which linked the reducing power generated by illuminated spinach chloroplasts to the Azotobacter nitrogen-fixing enzyme complex. The photoreduction of the yellow azotoflavin by chloroplasts produced a stable, free-radical semiquinone, blue in color, with properties similar to those described by other investigators for an Azotobacter flavoprotein of unknown biological function.