Purification of proteolipid protein and production of specific antiserum.

Journal of neuroimmunology

PubMedID: 2419357

Hampson DR, Poduslo SE. Purification of proteolipid protein and production of specific antiserum. J Neuroimmunol. 1986;11(2):117-29.
Proteolipid protein, the major protein in CNS myelin, was purified using several chromatographic steps. No detergents were used. Chromatography in organic solvents on lipophilic Sephadex (LH-60) for delipidation, on ion exchange chromatography for protein separation, and again on lipophilic Sephadex (LH-20) for desalting, produced homogeneous preparations of proteolipid protein. Rabbit antibodies to proteolipid protein were produced after 2 injections of a total of 500 micrograms of protein. By immunotransfer and absorption experiments, it was found that the antiserum bound to proteolipid protein and a closely related protein, DM-20; it did not bind to basic protein or other myelin proteins.