Large scale, rapid purification of recombinant tissue-type plasminogen activator.

FEBS letters

PubMedID: 3100325

Dodd I, Jalalpour S, Southwick W, Newsome P, Browne MJ, Robinson JH. Large scale, rapid purification of recombinant tissue-type plasminogen activator. FEBS Lett. 1986;209(1):13-7.
Recombinant tissue-type plasminogen activator (rt-PA) from cultures of a genetically manipulated Bowes melanoma cell line (TRBM6) was purified in batches of average volume 451 using an autoclavable, reusable, continuous chromatography system comprising zinc chelate-Sepharose CL4B and lysine-Sepharose CL4B. After eight successive purifications the rt-PA was ultrafiltered to yield a preparation containing 4.9 mg protein/ml and 2.7 X 10(6) IU/ml. Analysis by SDS-polyacrylamide gel electrophoresis followed by staining with Coomassie brilliant blue R250 showed major protein bands at Mr = 63,000 and 65,000; most of the material was in the 1-chain form. The potential usefulness of a simple, rapid continuous chromatography system that can be operated under aseptic conditions is discussed.