Nanochannel-based electrochemical assay for transglutaminase activity.

Chemical communications (Cambridge, England)

PubMedID: 25233491

Fernández I, Sánchez A, Díez P, Martínez-Ruiz P, Di Pierro P, Porta R, Villalonga R, Pingarrón JM. Nanochannel-based electrochemical assay for transglutaminase activity. Chem Commun (Camb). 2014;.
A novel electrochemical assay to quantify transglutaminase activity is reported. The assay is based on the enzyme-controlled diffusion of Fe(CN)6(3-/4-) through amino-functionalized nanochannels of a mesoporous silica thin film on a Au surface in the presence of N-benzyloxycarbonyl-l-glutaminylglycine.