Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.

Proceedings of the National Academy of Sciences of the United States of America

PubMedID: 4905990

Takebe I, Otsuki Y. Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc Natl Acad Sci USA. 1969;64(3):843-8.
It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.