Identification of polysomal RNA in BHK cells infected by sindbis virus.

Journal of virology

PubMedID: 4633684

Mowshowitz D. Identification of polysomal RNA in BHK cells infected by sindbis virus. J Virol. 1973;11(4):535-43.
Polysomes were prepared from Sindbis virus-infected BHK cells. The major species of RNA in these polysomes was identified as 26S RNA (interjacent RNA) by (i) disrupting the polysomes with EDTA; (ii) treating the infected cells with puromycin; and (iii) isolating polysomes from cells infected with a temperature-sensitive mutant that does not form nucleocapsids. Small amounts of 42S RNA and 33S RNA were also found in polysomes.