X chromosome inactivaton and SV40 transformation of mammalian cells.

Somatic cell genetics

PubMedID: 232578

Raskind WH, Gartler SM. X chromosome inactivaton and SV40 transformation of mammalian cells. Somatic Cell Genet. 1979;5(6):945-55.
Five embryonic mouse cultures and one human fibroblast culture were transformed with SV40. The cultures were studied cytologically to see if the normal pattern of sex chromosome replication was maintained in SV40 transformed cells. Characteristic late replication patterns were observed for both the X and Y chromosomes, and there was no evidence for loss of the inactive X chromosome, even in cells with 4 or more X chromosomes. The human line was heterozygous at two X-linked loci and a clonal analysis showed that the expression of X-linked genes was not affected by SV40 transformation.