Nuclear magnetic resonance studies of the structure and binding sites of enzymes. X. Preparation of selectively deuterated analogs of staphylococcal nuclease.

Proceedings of the National Academy of Sciences of the United States of America

PubMedID: 5271760

Putter I, Barreto A, Markley JL, Jardetzky O. Nuclear magnetic resonance studies of the structure and binding sites of enzymes. X. Preparation of selectively deuterated analogs of staphylococcal nuclease. Proc Natl Acad Sci USA. 1969;64(4):1396-403.
Five different selectively deuterated analogs of staphylococcal nuclease (Foggi) were prepared having all but a selected few hydrogen atoms in the enzyme molecule replaced by deuterium. The (1)H-NMR spectra of these analogs are extremely simplified, since they consist of lines arising only from the remaining protonated sites.