Synthesis of vaccinia viral proteins in cytoplasmic extracts. II. Identification of early and late viral proteins.

Journal of virology

PubMedID: 5360521

Moss B, Katz E. Synthesis of vaccinia viral proteins in cytoplasmic extracts. II. Identification of early and late viral proteins. J Virol. 1969;4(5):596-602.
The synthesis of vaccinia viral proteins has been studied in a cell-free system prepared from vaccinia virus-infected HeLa cells. The radioactively labeled proteins were identified as viral proteins by immunodiffusion, disc gel electrophoresis, and disc gel-immunoelectrophoresis. The cytoplasmic extracts, obtained from infected cells at different times during viral replication, synthesized the corresponding "early" or "late" viral proteins.