Development of peptidyl lysine dendrons: 1,3-diploare cycloaddition for peptide coupling and antibody recognition.

Chemical biology & drug design

PubMedID: 25290586

Hüttl C, Hettrich C, Riedel M, Henklein P, Rawel H, Bier FF. Development of peptidyl lysine dendrons: 1,3-diploare cycloaddition for peptide coupling and antibody recognition. Chem Biol Drug Des. 2014;.
A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13-DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N-terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid phase peptide synthesis (SPPS) allows preparing the peptide dendron in high purity and establishing the possibility of automation. The presented peptide dendron is a promising candidate as multivalent ligand and was used for antibody B13-DE1 recognition. The binding affinity increases with higher dendron generation without loss of specificity. The analysis of biospecific interaction between the synthesized peptide dendron and the antibody was done via surface plasmon resonance (SPR) technique. The presented results show a promising tool for investigations of antigen-antibody reactions. This article is protected by copyright. All rights reserved.