Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium: it enables the molecular investigation of dimorphism.

Yeast (Chichester, England)

PubMedID: 25308606

Papp L, Sipiczki M, Holb IJ, Miklós I. Optimal conditions for mycelial growth of Schizosaccharomyces japonicus cells in liquid medium: it enables the molecular investigation of dimorphism. Yeast. 2014;.
The non-pathogenic dimorphic fission yeast, Schizosaccharomyces japonicus could be a suitable model organism for investigation of the genetic background of mycelial growth, as it has haploid chromosome set and its genome is sequenced. Since earlier results have suggested that its morphological transition required solid substrates, but molecular biological experiments would require hyphae production in a liquid medium, we wanted to find circumstances which enable hyphae production in liquid media. Several external conditions were investigated, but the strongest inducer was Fetal Bovine Serum. Its positive effect could be hampered by heat and was dependent on pH, temperature and concentration of the serum. Other protein containing compounds such as peptone and BSA or amino acids proved to be ineffective or weak. Generally, the uninduced and induced mycelial growth of Sch. japonicus could be improved by lower external pH and higher temperature. This article is protected by copyright. All rights reserved.