Evolutionary and bioinformatics analysis of the spike glycoprotein gene of H120 vaccine strain protectotype of infectious bronchitis virus from India.

Biotechnology and applied biochemistry

PubMedID: 25311758

Kamble NM, S A, Gaikwad SS, Shukla SK, Khulape SA, Dey S, Mohan CM. Evolutionary and bioinformatics analysis of the spike glycoprotein gene of H120 vaccine strain protectotype of infectious bronchitis virus from India. Biotechnol Appl Biochem. 2014;.
Infectious bronchitis virus (AIB) causative agent of avian infectious bronchitis, is an important disease that produces severe economic losses in the poultry industry worldwide. Recent AIB outbreaks in India have been associated with poor growth in broilers, drop in egg production and thin egg shells in layers. The complete spike gene of Indian AIB vaccine strain was amplified and sequenced using conventional RT-PCR and submitted in GenBank (Accession no KF188436). Phylogenetic analysis revealed that vaccine strain currently used Belongs to H120 genotype an attenuated strain of Massachusetts (Mass) serotype. Nucleotide and amino acid sequence comparisons have shown that the reported spike gene from Indian isolates bear 71.8 to 99% and 71.4% to 96.9% genetic similarity with H120 strain sequenced. The study demonstrates a identity of live attenuated IBV vaccine strain routinely used for the first time. Based on nucleotide and amino acid relatedness studies of vaccine strain with reported IBV sequences from India it is shown that current vaccine strain is efficient for controlling the IBV infection. Continuous monitoring of IBV outbreaks by sequencing for genotyping and in vivo cross protection studies for serotyping is not only important for epidemiological investigation but also for evaluation of efficacy of current vaccine used. This article is protected by copyright. All rights reserved.