Investigation of membrane protein-protein interactions using correlative FRET-PLA.

BioTechniques

PubMedID: 25312088

Ivanusic D, Eschricht M, Denner J. Investigation of membrane protein-protein interactions using correlative FRET-PLA. BioTechniques. 2014;57(4):188-98.
Fluorescence resonance energy transfer (FRET) analysis and the recently developed proximity ligation assay (PLA) are widely used to study protein-protein interactions in situ. We have developed correlative FRET-PLA to monitor interactions between membrane proteins that frequently cause problems in confirmatory co-immunoprecipitation assays. Correlative FRET-PLA is particularly aimed at delivering robust and reliable results and is useful for investigating protein-protein interactions.