Large-scale label-free phosphoproteomics: from technology to data interpretation.

Bioanalysis

PubMedID: 25384593

Guo H, Isserlin R, Lugowski A, Kuzmanov U, Emili A. Large-scale label-free phosphoproteomics: from technology to data interpretation. Bioanalysis. 2014;6(18):2403-20.
Protein phosphorylation plays a central role in the dynamic intracellular signaling and the control of biochemical pathways in all living cells. Recent advances in high-performance MS/MS-based technology make the large-scale identification and quantification of phosphorylation sites possible. Here, we review the full data generation pipeline, starting from sample preparation methods and LC-MS detection procedures, through to data processing and analysis software tools that facilitate the systematic comparative profiling of thousands of phosphoproteins in different biological specimens in a single experiment. We emphasize current challenges and promising avenues for the mechanistic interpretation and visualization of global phosphorylation networks and their relevance to human health and disease.