Metallosphaera tengchongensis sp. nov., a novel species of acidothermophilic archaeon isolated from a hot spring.

International journal of systematic and evolutionary microbiology

PubMedID: 25404480

Peng TJ, Liu LJ, Liu C, Yang ZF, Liu SJ, Jiang CY. Metallosphaera tengchongensis sp. nov., a novel species of acidothermophilic archaeon isolated from a hot spring. Int J Syst Evol Microbiol. 2014;.
Two novel acidothermophilic archaea, strain Ric-A(T) and Ric-F, were isolated from the muddy water samples of a sulfuric hot spring located at Tengchong County, Yunnan Province, China. They were aerobic and facultatively chemolithoautotrophic. Both strains could oxidize S(0) and K2S4O6 for autotrophic growth, and also could use organic materials for heterotrophic growth. Growth was observed at 55-75 °C and pH 1.5-6.5. The strains could oxidize metal sulfide ores, showing their potential in bioleaching. The DNA G+C content of Ric-A(T) and Ric-F were 41.8 and 41.6 mol%, respectively. Analysis of 16S rRNA gene sequences showed that the two strains share 99.8% sequence similarity to each other, but < 97% to the other known species of the genus Metallosphaera. DNA-DNA hybridization indicated that the isolates were different strains of a novel species of the genus Metallosphaera. Strains Ric-A(T) and Ric-F also shared a number of physiological and biochemical characteristics that distinguished them from the recognized species of the genus Metallosphaera. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with their relatives, it was concluded that the strains Ric-A(T) and Ric-F represent a novel species of the genus Metallosphaera, for which the name Metallosphaera tengchongensis is proposed. The type strain is Ric-A(T) (=NBRC109472(T)=CGMCC1.12287(T)).