Identity of V factor in culture medium used for prior growth of two strains of Staphylococcus aureus.

International journal of systematic bacteriology

PubMedID: 1390114

Niven DF, Le Blanc L. Identity of V factor in culture medium used for prior growth of two strains of Staphylococcus aureus. Int J Syst Bacteriol. 1992;42(4):642-4.
Staphylococcus aureus ATCC 12598 and ATCC 25923 were starved of pyridine nucleotides and precursors and then grown in a semidefined medium containing [carbonyl-14C]nicotinamide. Samples of medium from late-exponential-phase and stationary-phase cultures were analyzed for 14C-metabolites. In all cases, V factor was present primarily as NAD.