Unusual 2(1H)-pyrazinones isolated from a culture of a Brazilian marine-derived Streptomyces sp.

Natural product communications

PubMedID: 25918790

Thomasi SS, Granato AC, Romano LH, Dhooghe L, do Nascimento ES, Badino AC, da Silva MF, Ferreira AG, VenĂ¢ncio T. Unusual 2(1H)-pyrazinones isolated from a culture of a Brazilian marine-derived Streptomyces sp. Nat Prod Commun. 2014;9(9):1275-8.
Four new secondary metabolites, giovaninones A-D (1-4), were isolated from an ethyl acetate extract of a culture of a marine-derived Streptomyces strain designated SS99BA-2. Chemical analysis was completely conducted in a coupled automated LC-SPE system with the use of a cryogenic NMR probehead and HRMS. The application of this system to identify, purify and elucidate all the structures is described.