PERMANENT GENETIC RESOURCES: Twelve polymorphic microsatellite markers for the bonefish, Albula vulpes and two congeners.

Molecular ecology resources

PubMedID: 21585790

Seyoum S, Wallace EM, Tringali MD. PERMANENT GENETIC RESOURCES: Twelve polymorphic microsatellite markers for the bonefish, Albula vulpes and two congeners. Mol Ecol Resour. 2008;8(2):354-6.
Twelve polymorphic microsatellite loci were isolated for the bonefish, Albula vulpes using a polymerase chain reaction-based procedure. The number of alleles ranged from two to 23 (mean = 8. 8) in 37 specimens from south Florida. Observed and expected heterozygosities ranged from 0. 07 to 0. 77 (mean = 0. 42) and from 0. 07 to 0. 84 (mean = 0. 48), respectively. There were no significant departures from Hardy-Weinberg equilibrium and no evidence of genotypic disequilibrium between any pair of loci. In a cross-amplification test, all markers yielded appropriately sized alleles for specimens of the provisional Albula sp. B and 11 of the 12 loci amplified for those of Albula glossodonta.