Development of polymorphic microsatellite markers for the dung fly (Sepsis cynipsea).

Molecular ecology resources

PubMedID: 21564957

Greminger MP, Schäfer MA, Nater A, Blanckenhorn WU, Krützen M. Development of polymorphic microsatellite markers for the dung fly (Sepsis cynipsea). Mol Ecol Resour. 2009;9(6):1554-6.
The polyandrous fly Sepsis cynipsea has been used extensively in studies of sexual selection and local adaptation. We isolated and characterized 11 novel microsatellite markers for S. cynipsea from a genomic library and screened 32 flies for polymorphism. All microsatellite markers show high allelic diversity with an average of 9. 64 alleles per locus. Two microsatellites were found likely to be X-linked. These novel markers will significantly advance studies of sexual selection and evolutionary genetics of S. cynipsea and related species, especially given the low numbers of markers currently available in this family.