Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for lysozyme purification from chicken egg white.

Artificial cells, nanomedicine, and biotechnology (Print)

PubMedID: 23110388

Köse K, Denizli A. Poly(hydroxyethyl methacrylate) based magnetic nanoparticles for lysozyme purification from chicken egg white. Artif Cells Nanomed Biotechnol. 2013;41(1):13-20.
The purpose of this article is to synthesize poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-tryptophan) [mPHEMATrp] magnetic nanoparticles for lysozyme purification from chicken egg white. mPHEMATrp nanoparticles (38 nm in diameter) were synthesized by surfactant-free emulsion polymerization. Specific surface area of the mPHEMATrp nanoparticles was calculated to be 896 m2/g. Elemental analysis of mPHEMATrp nanoparticles was estimated in the range of 5. 7-48. 3 µmol MATrp/g. The maximum lysozyme adsorption capacity of the mPHEMATrp nanoparticles was 376. 1 mg/g. The mPHEMATrp nanoparticles could be used five times without decreasing the lysozyme adsorption capacity significantly. THE RESULTS
indicate that the mPHEMATrp nanoparticles promise high selectivity for lysozyme purification.