A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.

G3 (Bethesda, Md.)

PubMedID: 24727291

Ucisik-Akkaya E, Leatherwood JK, Neiman AM. A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe. G3 (Bethesda). 2014;4(6):1173-82.
Yeast sporulation is a highly regulated developmental program by which diploid cells generate haploid gametes, termed spores. To better define the genetic pathways regulating sporulation, a systematic screen of the set of ~3300 nonessential Schizosaccharomyces pombe gene deletion mutants was performed to identify genes required for spore formation. A high-throughput genetic method was used to introduce each mutant into an h(90) background, and iodine staining was used to identify sporulation-defective mutants. The screen identified 34 genes whose deletion reduces sporulation, including 15 that are defective in forespore membrane morphogenesis. In S. pombe, the total number of sporulation-defective mutants is a significantly smaller fraction of coding genes than in S. cerevisiae, which reflects the different evolutionary histories and biology of the two yeasts.