Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug.

Nanoscale

PubMedID: 25572921

Chen HH, Yuan H, Cho H, Sosnovik DE, Josephson L. Cytoprotective nanoparticles by conjugation of a polyhis tagged annexin V to a nanoparticle drug. Nanoscale. 2015;7(6):2255-9.
We synthesized a cytoprotective magnetic nanoparticle by reacting a maleimide functionalized Feraheme (FH) with a disulfide linked dimer of a polyhis tagged annexin V. Following reductive cleavage of disulfide, the resulting annexin-nanoparticle (diameter = 28. 0 ± 2. 0 nm by laser light scattering, 7. 6 annexin's/nanoparticle) was cytoprotective to cells subjected to plasma membrane disrupting chemotherapeutic or mechanical stresses, and significantly more protective than the starting annexin V. Annexin-nanoparticles provide an approach to the design of nanomaterials which antagonize the plasma membrane permeability characteristic of necrosis and which may have applications as cytoprotective agents.