[Differential protein expressions in breast cancer between drug sensitive tissues and drug resistant tissues].

Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences

PubMedID: 23456065

Yi W, Peng J, Zhang Y, Fu F, Zou Q, Tang Y. [Differential protein expressions in breast cancer between drug sensitive tissues and drug resistant tissues]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2013;38(2):148-54.
OBJECTIVE
To investigate the differential expression of the sensitive and resistant relative proteins in human breast cancer tissue.

METHODS
A drug sensitive group and a drug resistant group for chemotherapy in patients with breast cancer were selected through neoadjuvant. The differential protein expression in 2 groups was detected by proteomics techniques, and parts of differential proteins were identified by Western blot.

RESULTS
There were 13 differential proteins in the 2 groups, in which the expression of 3 proteins was up-regulated and 10 down-regulated. Seven proteins were identified by Western blot. The expression of keratin type I cytoskeletal 19 (KIC19), thymidine phosphorylase (TYPH) was upregulated, and the expression of heat shock protein 27 (HSP27), keratin type I cytoskeletal 9 (KIC9), collagen alpha-2(VI) (CO6A2), vimentin (VIME), and actin cytoplasmic 1 (ACTB) was down-regulated in the drug resistant group. There was significant difference between the 2 groups (P<0.01).

CONCLUSION
The expression of KIC19 and TYPH may be correlated with drug resistance in patients with breast cancer, and HSP27, KIC9, CO6A2, VIME, and ACTB may be correlated with drug sensitivity.