Effect of ASCs on the graft survival rates of fat particles in rabbits.

Journal of plastic surgery and hand surgery

PubMedID: 23210496

He X, Zhong X, Ni Y, Liu M, Liu S, Lan X. Effect of ASCs on the graft survival rates of fat particles in rabbits. J Plast Surg Hand Surg. 2013;47(1):3-7.
This study was conducted to explore the effect of adipose-derived stem cells (ASCs) on the graft survival rates of fat particles in rabbits. Six domestic rabbits were used for a 3-month study; 1. 4 grams of fat tissues were harvested from the bilateral inguinal regions of each animal. They were cut into granules and divided into three parts (A = 0. 4 g, B = 0. 4 g, and C = 0. 6 g). Part A was centrifuged after 0. 075% collagenase digestion for isolation of the stromal vascular fraction (SVF). About 0. 2 grams of SVF containing ASCs was obtained, and then incorporated with part B to create a treated group, whereas part C was treated as a control group. The tissues in both groups were randomly transplanted into a subcutaneous space that had been created on each side of the dorsal midline of the rabbit. The grafts were taken out after 3 months for calculation of the survival rates. The graft survival rate in the treated group was 23. 56 ± 2. 49%, while that in the control group was 11. 06 ± 2. 10%. The graft survival rate in the treated group increased significantly, compared with in the control group (p < 0. 01). Improved transplantation effects may be obtained by implanting the fat particles mixed with ASCs. It is suggested that this approach has the potential for becoming a new method of fat graft in clinical practice.