Association of calcium urolithiasis with urokinase P141L and 3'-UTR C>T polymorphisms in a Japanese population.

Urolithiasis

PubMedID: 23532423

Hagikura S, Wakai K, Kawai S, Goto Y, Naito M, Hagikura M, Gotoh M, Hamajima N. Association of calcium urolithiasis with urokinase P141L and 3'-UTR C>T polymorphisms in a Japanese population. Urolithiasis. 2013;41(1):47-52.
This was a case-control study to analyze the associations between calcium urolithiasis and the urokinase polymorphisms, P141L (rs2227564) and 3'-UTR C>T (rs4065), in a Japanese population. CASES
consisted of 232 patients with urinary calcium stones (174 men and 58 women) who presented to a general hospital between April 2009 and June 2011.Among these cases, 115 (49. 6 %) patients had calcium oxalate stones alone, and 113 (48. 7 %) patients had calcium oxalate stones mixed with calcium phosphate stones.

CONTROLS
consisted of 454 subjects who had a routine health check-up in the same prefecture.The two polymorphisms were genotyped via polymerase chain reaction with confronting two-pair primers. In the control group, the genotype frequencies of P141L were 0. 573 for PP, 0. 375 for PL, and 0. 052 for LL, and those of 3'-UTR C>T were 0. 835 for CC, 0. 165 for CT, and TT was not identified. Neither of the polymorphisms was significantly associated with urolithiasis. The age- and sex-adjusted odds ratios of urolithiasis were 0. 96 [95 % confidence interval (CI), 0. 66-1. 41] for PL and 1. 22 (0. 58-2. 57) for LL as compared with PP genotype of P141L, and 1. 01 (0. 62-1. 64) for CT as compared with CC genotype of 3'-UTR C>T. When compared with the PP genotype of P141L, the frequency of PL was significantly lower in female cases with a family history of urolithiasis than in females without such family history (p = 0. 028). P141L and 3'-UTR polymorphisms of the urokinase gene are not associated with urolithiasis in a Japanese population.