Traction microscopy to identify force modulation in subresolution adhesions.

Nature methods

PubMedID: 26030446

Han SJ, Oak Y, Groisman A, Danuser G. Traction microscopy to identify force modulation in subresolution adhesions. Nat Methods. 2015;.
We present a reconstruction algorithm that resolves cellular tractions in diffraction-limited nascent adhesions (NAs). The enabling method is the introduction of sparsity regularization to the solution of the inverse problem, which suppresses noise without underestimating traction magnitude. We show that NAs transmit a distinguishable amount of traction and that NA maturation depends on traction growth rate. A software package implementing this numerical approach is provided.