Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

PubMedID: 26165297

Kanno Y, Ino K, Inoue KY, Suda A, Kunikata R, Matsudaira M, Shiku H, Matsue T. Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity. Anal Sci. 2015;31(7):715-9.
In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 ┬Ám. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.